# 📐 L2 Spec — RNA-seq Cell-Type Classification (PWDR) — Spec Skeleton
**ID:** `L2-520-001` · **Status:** ⊙ Testnet (genesis catalog)
**Parents:** ⬆ Principle [`L1-520`](/artifact/l1/L1-520)
> **🎯 Tolerance (ε):** expression
> **⚖ Difficulty score:** 0.5 · **Delta tier:** 5
> **⛓ On-chain block:** 41555320
---
## 1. Domain Ω
**Type:** `range`
| Parameter | Unit | Range |
|---|---|---|
| N cells | — | 100 – 1000000 |
| N genes | — | 1000 – 60000 |
| Batch effect | — | [NEEDS_HAND_AUTHORING: bounds] |
| Dropout rate | — | 0.0 – 0.5 |
| Reads per cell | — | 1000 – 1000000 |
| Sequencing depth | — | [NEEDS_HAND_AUTHORING: bounds] |
| Library complexity | — | [NEEDS_HAND_AUTHORING: bounds] |
| Doublet contamination | — | [NEEDS_HAND_AUTHORING: bounds] |
| Taxonomy disagreement | — | [NEEDS_HAND_AUTHORING: bounds] |
| Ambient rna contamination | — | [NEEDS_HAND_AUTHORING: bounds] |
| Marker panel coverage uncertainty | — | [NEEDS_HAND_AUTHORING: bounds] |
## 2. Tolerance (ε)
**Type:** `fn`
```
0.85_accuracy
```
## 3. Difficulty
| Property | Value |
|---|---|
| Difficulty score | 0.5 |
| Delta tier | 5 |
## 4. Fingerprint
`8e9fd35117f0114d4b71c97c478be3505a57bff651a7f20402f32b9b08357ec9`
## 5. On-chain Registration
- **Chain hash:** `0x93455f2f4f8be7e6a64a6811177253e8fb3b05a0c95f2604212e629c13e7662d`
- **Chain tx hash:** `0xe27e26dd15a4af43b0cf388a64832446d1be040e482e6dee5872ee4b7a207c07`
- **Chain block:** `41555320`
---
## File Mapping
This bundle consists of: `L2-520-001.md`, `L2-520-001.json`.
| File | Role | How to regenerate |
|------|------|-------------------|
| `L2-520-001.md` | Source of truth — edit this | Human or LLM |
| `L2-520-001.json` | Structured metadata for the registry | LLM regenerates from the sections above |
**Prompt for your LLM after editing this Markdown:**
> Read the attached Markdown. Regenerate the sibling `.json` so every field matches.
> Preserve the schema documented in the rows above.
> Output each file in its own fenced code block tagged with the filename.
> Output only the JSON object.
_This Markdown was auto-synthesized from the catalog row for `L2-520-001`._
_Edit it, regenerate the JSON, and submit at [/submit](/submit) to claim the artifact._