# 📐  L2 Spec — Cryo-EM SPA Homogeneous Spec

**ID:** `L2-129-001` · **Status:** ⊙ Testnet (genesis catalog)
**Parents:** ⬆ Principle [`L1-129`](/artifact/l1/L1-129)

> **🎯 Tolerance (ε):** expression
> **⚖ Difficulty score:** 0.58 · **Delta tier:** 5
> **⛓ On-chain block:** 41554217

---

## 1. Domain Ω

**Type:** `range`

| Parameter | Unit | Range |
|---|---|---|
| Dose | — | 10 – 100 |
| N particle | — | 1000 – 10000000 |
| Defocus um | µm | 0.3 – 5.0 |
| Pixel angstrom | — | 0.5 – 3.0 |
| Ctf miscalibration | — | 0.0 – 500 |
| Per particle motion | — | 0.0 – 5 |
| Pose estimate error | — | 0.0 – 10 |
| Preferential orientation | — | 0.0 – 0.8 |
| Conformational heterogeneity | — | 0.0 – 1.0 |

## 2. Tolerance (ε)

**Type:** `fn`

```
2.5 + 1.0 * log10(100000/N_particle) + 0.2 * pose_estimate_error + 5.0 * conformational_heterogeneity + 2.0 * preferential_orientation
```

## 3. Difficulty

| Property | Value |
|---|---|
| Difficulty score | 0.58 |
| Delta tier | 5 |

## 4. Fingerprint

`36b48dc890f18b32e4575886ec2d9a970238de5b88c8c5eb91552c372580f8ed`

## 5. On-chain Registration

- **Chain hash:** `0xcd0d5cb55acb66728d5f20275d0ffc2c75df092e322fdc21179cf1189439c474`
- **Chain tx hash:** `0x380ba6f72e1173ebae7e179d3938d5c04fb9a2a07a8cbaea259204b04019694f`
- **Chain block:** `41554217`

---

## File Mapping

This bundle consists of: `L2-129-001.md`, `L2-129-001.json`.

| File | Role | How to regenerate |
|------|------|-------------------|
| `L2-129-001.md` | Source of truth — edit this | Human or LLM |
| `L2-129-001.json` | Structured metadata for the registry | LLM regenerates from the sections above |

**Prompt for your LLM after editing this Markdown:**

> Read the attached Markdown. Regenerate the sibling `.json` so every field matches.
> Preserve the schema documented in the rows above.
> Output each file in its own fenced code block tagged with the filename.
> Output only the JSON object.

_This Markdown was auto-synthesized from the catalog row for `L2-129-001`._
_Edit it, regenerate the JSON, and submit at [/submit](/submit) to claim the artifact._