# 📐  L2 Spec — DNA-PAINT (transient DNA-hybridization localization microscopy) Mismatch-Only Spec

**ID:** `L2-024-001` · **Status:** ⊙ Testnet (genesis catalog)
**Parents:** ⬆ Principle [`L1-024`](/artifact/l1/L1-024)

> **🎯 Tolerance (ε):** expression
> **⚖ Difficulty score:** 0.45 · **Delta tier:** 5
> **⛓ On-chain block:** 41554172

---

## 1. Domain Ω

**Type:** `range`

| Parameter | Unit | Range |
|---|---|---|
| H | px | 64 – 2048 |
| W | px | 64 – 2048 |
| Na | — | 1.3 – 1.49 |
| T frames | — | 5000 – 200000 |
| Pixel nm | nm | 80 – 300 |
| Drift nm per frame | — | 0.0 – 3.0 |
| Photons per binding | — | 500 – 50000 |
| Non specific binding | — | 0.0 – 0.2 |
| Strand concentration nm | nm | 0.5 – 50 |

## 2. Tolerance (ε)

**Type:** `fn`

```
4.0 + 3.0 * log10(photons_per_binding / 500) - 2.0 * drift_nm_per_frame - 10.0 * non_specific_binding
```

## 3. Difficulty

| Property | Value |
|---|---|
| Difficulty score | 0.45 |
| Delta tier | 5 |

## 4. Fingerprint

`f7b955c5a919e5bf1998873552731ebf5905ea46315141ec52b49c175f22a534`

## 5. On-chain Registration

- **Chain hash:** `0xa45fe33fdbf05cb7beb830ea84a522d68de53163ea58966cbdaeb16a97bb3a93`
- **Chain tx hash:** `0x4b11f704f3c51cedf2f3261909ad873fd82da43c43376e2b954cf2cd8f481a97`
- **Chain block:** `41554172`

---

## File Mapping

This bundle consists of: `L2-024-001.md`, `L2-024-001.json`.

| File | Role | How to regenerate |
|------|------|-------------------|
| `L2-024-001.md` | Source of truth — edit this | Human or LLM |
| `L2-024-001.json` | Structured metadata for the registry | LLM regenerates from the sections above |

**Prompt for your LLM after editing this Markdown:**

> Read the attached Markdown. Regenerate the sibling `.json` so every field matches.
> Preserve the schema documented in the rows above.
> Output each file in its own fenced code block tagged with the filename.
> Output only the JSON object.

_This Markdown was auto-synthesized from the catalog row for `L2-024-001`._
_Edit it, regenerate the JSON, and submit at [/submit](/submit) to claim the artifact._