# 📐  L2 Spec — Total Internal Reflection Fluorescence (TIRF) Mismatch-Only Spec

**ID:** `L2-012-001` · **Status:** ⊙ Testnet (genesis catalog)
**Parents:** ⬆ Principle [`L1-012`](/artifact/l1/L1-012)

> **🎯 Tolerance (ε):** expression
> **⚖ Difficulty score:** 0.45 · **Delta tier:** 5
> **⛓ On-chain block:** 41554158

---

## 1. Domain Ω

**Type:** `range`

| Parameter | Unit | Range |
|---|---|---|
| H | px | 128 – 4096 |
| W | px | 128 – 4096 |
| Na | — | 1.4 – 1.49 |
| D p nm | nm | 50 – 300 |
| Pixel nm | nm | 50 – 300 |
| Peak photons | photons | 50 – 5000 |
| Coverslip angle drift | — | 0.0 – 0.5 |
| Penetration depth error | — | 0.0 – 0.3 |
| Background volume fluorescence | — | 0.0 – 0.3 |

## 2. Tolerance (ε)

**Type:** `fn`

```
22.0 + 1.8 * log10(peak_photons / 50) - 5.0 * coverslip_angle_drift - 8.0 * background_volume_fluorescence
```

## 3. Difficulty

| Property | Value |
|---|---|
| Difficulty score | 0.45 |
| Delta tier | 5 |

## 4. Fingerprint

`6fd60aeabb4e3d9a066bd662d1c4e246edbb39f79e331936cd339d28eaf27a7d`

## 5. On-chain Registration

- **Chain hash:** `0xab485e7abb850b3cee2943a2a9988f43ac8ec522c0850b5b6ad2317f5ee0dd3a`
- **Chain tx hash:** `0xc03bcd18ba7fcfc3e27ec3d156507b50cab3f3e35be6ae55f482a9cc9b8067ed`
- **Chain block:** `41554158`

---

## File Mapping

This bundle consists of: `L2-012-001.md`, `L2-012-001.json`.

| File | Role | How to regenerate |
|------|------|-------------------|
| `L2-012-001.md` | Source of truth — edit this | Human or LLM |
| `L2-012-001.json` | Structured metadata for the registry | LLM regenerates from the sections above |

**Prompt for your LLM after editing this Markdown:**

> Read the attached Markdown. Regenerate the sibling `.json` so every field matches.
> Preserve the schema documented in the rows above.
> Output each file in its own fenced code block tagged with the filename.
> Output only the JSON object.

_This Markdown was auto-synthesized from the catalog row for `L2-012-001`._
_Edit it, regenerate the JSON, and submit at [/submit](/submit) to claim the artifact._