{"artifact_id":"L1-012","layer":"L1","title":"Total Internal Reflection Fluorescence (TIRF)","domain":"Microscopy","sub_domain":"Evanescent-field surface-selective imaging","physics_fingerprint":{"L_DAG":2.5,"title":"Total Internal Reflection Fluorescence (TIRF)","domain":"Microscopy","carrier":"photon","primitives":["L.excitation.evanescent","K.psf.airy","int.temporal"],"sub_domain":"Evanescent-field surface-selective imaging","noise_model":"poisson_gaussian","problem_class":"linear_inverse","solution_space":"2D_intensity","difficulty_delta":3,"integration_axis":"temporal","sensing_mechanism":"evanescent_field_excitation"},"observable_profile":{"metric":"PSNR_dB","regime":"Existence of the recovered 2D intensity is guaranteed within the declared Omega bounds. Uniqueness holds on the measurement-supported subspace; out-of-support modes are controlled by the declared priors. Stability is well-conditioned (kappa_eff ~= 8); coverslip_angle_drift dominates the stability cliff; penetration_depth_error and the remaining mismatch parameters contribute higher-order bias terms. Poisson signal noise + gaussian read noise sets the irreducible data-fidelity floor, while mild Tikhonov or analytic inversion is sufficient at the nominal Omega point.","secondary":"SSIM"},"size_tiers":{"center_spec":{"omega":{"H":512,"W":512,"NA":1.49,"d_p_nm":100,"pixel_nm":100,"peak_photons":500,"coverslip_angle_drift":0.0,"background_volume_fluorescence":0.0},"input_format":"measurement_only","problem_class":"tirf","forward_operator":"tirf_forward","epsilon_fn_center":"28.0"},"omega_bounds":{"H":[128,4096],"W":[128,4096],"NA":[1.4,1.49],"d_p_nm":[50,300],"pixel_nm":[50,300],"peak_photons":[50,5000],"coverslip_angle_drift":[0.0,0.5],"penetration_depth_error":[0.0,0.3],"background_volume_fluorescence":[0.0,0.3]},"epsilon_bounds":{"psnr_db":[12.0,42.0]},"allowed_problem_classes":["tirf"],"allowed_omega_dimensions":["H","W","pixel_nm","NA","d_p_nm","peak_photons","coverslip_angle_drift","background_volume_fluorescence","coverslip_angle_drift","penetration_depth_error","background_volume_fluorescence"],"allowed_forward_operators":["tirf_forward"]},"hardness_fn":{"type":"epsilon_fn","delta":3,"kappa":160,"metric":"PSNR_dB"},"initiator_dataset":[{"name":"primary","weight":1.0,"ipfs_cid":null,"license_hash":null}],"status":"testnet","staked_pwm":0.0,"chain_hash":"0x6ea9b2675c3428287b42549754b99265fe3644e22a2ffc7338f9c6a9563c846a","chain_tx_hash":"0x6f7b2f941284af69ef41dac81758049fa90e84f27599413413a5c2a1d94f1a23","chain_block":41554155}